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Our TECHSPEC® Fluorescence Bandpass Filters perfectly match common fluorophores' absorption and emission characteristics in fluorescence imaging applications. Excitation and emission filters are available, featuring high transmissions in the passband (≥ 93% for VIS designs) and >OD 6 blocking outside of the passband. These filters are also ideal components in applications using spectroscopic fluorescence detection, including analytical chemistry and biomedical instruments such as DNA sequencers and polymerase chain reaction (qPCR) devices. Each filter features state-of-the-art hard-sputtered coatings and is mounted in a black anodized ring for easy system integration.
Common Specifications
Thickness Tolerance (mm):
±0.1
Construction:
Mounted in Black Anodized Ring
Physical Durability:
Adhesion per MIL-PRF-13830B, Section C.4.5.12 Moderate abrasion per MIL-PRF-13830B, Section C.4.5.11 Cleaning per MIL-C-48497A Section 4.5.4.2
Substrate Thickness (mm):
2.00 ±0.25
Angle of Incidence (°):
0 ±5
Optical Density OD (Average):
≥6.0
Coating:
Hard Coated
Surface Quality:
60-40
Transmitted Wavefront, RMS:
λ/4 (prior to coating)
Mount Thickness (mm):
3.5 ±0.1
Environmental Durability:
Humidity per MIL-STD-810H, Section 507.6 Temperature per MIL-STD-810H, Section 501.7 and 502.7
Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy. In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity.
Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences.
TECHSPEC® Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.
Technical Information
All mounted TECHSPEC® Optical Filters have an arrow on the side of the mount that points to the filter-coated surface for quick reference. Filter oriented such that arrow points to filter coated surface S1. Anti-reflective (AR) coating is applied to S2.
Edmund Optics offers comprehensive custom manufacturing services for optical and imaging components tailored to your specific application requirements. Whether in the prototyping phase or preparing for full-scale production, we provide flexible solutions to meet your needs. Our experienced engineers are here to assist—from concept to completion.
Custom Bandpass Filter using Shortpass and Longpass Filters
Do stock filters not meet your bandwidth or CWL requirements? Learn how custom bandpass filters are created using short or longpass filters at Edmund Optics.
New optical filters are being developed due to the advancements in applications and technologies. Learn more about the future of filters at Edmund Optics.
While working with machine vision, there are different types of filters that can be used to alter the image. Find out about the different types at Edmund Optics.
Fluorophores and Optical Filters for Fluorescence Microscopy
Want to know more about fluorophores and optical filters for fluorescence microscopy? Find out more information and in stock optical filters at Edmund Optics.
Nick Sischka explains why color consideration and illumination are so important for machine vision in this hands-on demonstration from the Edmund Optics 2021 Imaging Innovation Summit.
Confocal microscopy provides high resolution, elimination of out-of-focus glare due to spatial filtering, and reduction of light-induced damage to the sample.
Basic Principles of Raman Scattering and Spectroscopy
Raman spectroscopy is a technique used to identify the chemical composition of samples based on how light scatters off of them. Learn more & view related optics
A protective plastic coating protects a variety of our flat optics, such as optical windows, optical mirrors, and beamsplitters, from scratches during shipping.
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