494nm CWL, 50mm Dia, 20nm Bandwidth, OD 6 Fluorescence Filter

Product Family Description

• Common Wavelengths for Popular Fluorophores
• Excitation Filters and Emission Filters Available
• >93% Transmission
• >OD 6 Blocking, <3% from Edge of Bandwidth
• Fluorescence Filter Sets and Pre-Mounted Fluorescence Filter Cube Sets Also Available

Our TECHSPEC® Fluorescence Bandpass Filters perfectly match common fluorophores' absorption and emission characteristics in fluorescence imaging applications. Excitation and emission filters are available, featuring high transmissions in the passband (≥ 93% for VIS designs) and >OD 6 blocking outside of the passband. These filters are also ideal components in applications using spectroscopic fluorescence detection, including analytical chemistry and biomedical instruments such as DNA sequencers and polymerase chain reaction (qPCR) devices. Each filter features state-of-the-art hard-sputtered coatings and is mounted in a black anodized ring for easy system integration.

Fluorescence microscopy and spectroscopy applications typically use a combination of three filters for each color channel (detection of individual fluorophores), an excitation and an emission bandpass filter, as well as a dichroic filter (also dichroic beamsplitters, such as our Fluorescence Dichroic Filters and High-Performance Fluorescence Dichroic Filters). More information on fluorescence microscopy and an example beam path can be found in the AppNote Fluorophores and Optical Filters for Fluorescence Microscopy. In short, excitation filters “clean up” the excitation light used to excite the fluorophores in the sample. On the other hand, emission filters allow only the emitted fluorescence to pass through to the detector while effectively blocking the excitation light and different wavelengths, producing high-contrast images and superior image clarity.

Our fluorescence bandpass filters are engineered with cutting-edge technology and are designed to enhance signal-to-noise ratios, improve contrast, and deliver excellent performance in optical imaging applications. These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for various imaging applications, including widefield, confocal, multiphoton, and other fluorescence microscopy modalities. Whether you are imaging fixed cells, live cells, or tissues, our filters are engineered to deliver consistent and reliable image quality. Additionally, these filters can be used for fluorescence detection in qPCR, flow cytometry, or multi-well plate readers in applications such as immunofluorescence or high-throughput screening in which fluorescent molecules or dyes detect specific molecular structures or DNA sequences.

TECHSPEC® Fluorescence Bandpass Filters are optimized for maximum transmission within a specific wavelength range. They effectively isolate fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.